A diffusion-based microfluidic device for single-cell RNA-seq
نویسندگان
چکیده
منابع مشابه
Microfluidic device for single-cell analysis.
We have developed a novel microfluidic device constructed from poly(dimethylsiloxane) using multilayer soft lithography technology for the analysis of single cells. The microfluidic network enables the passive and gentle separation of a single cell from the bulk cell suspension, and integrated valves and pumps enable the precise delivery of nanoliter volumes of reagents to that cell. Various ap...
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Single cell RNA-seq (scRNA-seq) experiments suffer from a range of characteristic technical biases, such as dropouts (zero or near zero counts) and high variance. Current analysis methods rely on imputing missing values by various means of local averaging or regression, often amplifying biases inherent in the data. We present netSmooth, a network-diffusion based method that uses priors for the ...
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ژورنال
عنوان ژورنال: Lab on a Chip
سال: 2019
ISSN: 1473-0197,1473-0189
DOI: 10.1039/c8lc00967h